Lactate dehydrogenase in developing rat oral epithelium.
نویسندگان
چکیده
منابع مشابه
[Lactate dehydrogenase in human oral neoplastic tissues].
要 旨:エ ナメル上皮腫,歯 肉癌 および唾液腺腫瘍 の各種 口腔領 域上皮性腫瘍組織 につ いて,DNA量 あた りのLDH活 性 とLDH isoenzyme patternを 正常対 照組織 と比較 して検討 した。 LDH活 性 は,Wroblewski and LaDueの 方 法に準 じてNADHの 吸光度変化 に よ り測 定 した。LDH isoenzyme patternは,Dietz and Lubranoの 方 法に準 じてdisc電 気泳動 によ り分離 し,Ressler氏 法の 変法 による染色液 で酵素活性 を検 出 しdensitometryに よ り求 めた。 その結果,一 般 に腫瘍組織 においてはLDH活 性の上昇が認 め られ た。 エナメル上皮腫 のLDHisoenzyme patternはLDH-3にpeakが み られ,仔 牛 エナメル器...
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For the measurement of lactate dehydrogenase (LDH) isoenzyme biosynthesis a new method is presented which is based on the quantitative hybridization of isoenzyme MI with isoenzyme Hq. After reversible dissociation the formation of active LDH M4 was more rapid and occurred to a greater extent than the formation of LDH Hq. The formation of hybrid isoenzymes from the two subunits resulted in an in...
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For the measurement of lactate dehydrogenase (LDH) isoenzyme biosynthesis a new method is presented which is based on the quantitative hybridization of isoenzyme MI with isoenzyme Hq. After reversible dissociation the formation of active LDH M4 was more rapid and occurred to a greater extent than the formation of LDH Hq. The formation of hybrid isoenzymes from the two subunits resulted in an in...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1984
ISSN: 0022-1554,1551-5044
DOI: 10.1177/32.1.6690595